Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Trizma Base | 77-86-1 | MFCD00004679 | 100g
Trizma Base | Purity: 99% | Mol Wt: 121.14 | 77-86-1 | MFCD00004679 | 100g
Medchemexpress LLC 2-methylthioadenosine diphosphate trisodium | 475193-31-8 | 99.4% | 539.24 | 1 MG
2-Methylthioadenosine diphosphate trisodium is a potent purinergic P2Y receptor agonist, with EC50s of 19, 6.2, and 5 nM for human P2Y13, mouse P2Y13 and human P2Y12, respectively. It has pEC50s of 8.29 and 5.75 for human P2Y1 and rat P2Y6, respectively. It induces platelet aggregation and shape change, and inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1.
- Potent purinergic P2Y receptor agonist
- Acts on human P2Y13, mouse P2Y13, human P2Y12, human P2Y1 and rat P2Y6 receptors
- Induces platelet aggregation and shape change
- Inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1
Ethos Biosciences Buffer tris multipurpose classic 1/EA
Dilurex 1M Tris-HCL 10X Buffer is a classic laboratory buffer at pH 7 4 It does not include a saline ionic component and is free to be mixed with other components as needed in your laboratory kip the tedium of weighing powders and stocking raw materials that can expire Our buffer is convenient ready to use and will save you hours in the lab letting you focus on breakthroughs rather than buffer prep Thousands of researchers trust Ethos Biosciences buffers for their proven track record in top-tier publications and protocols - upgrade your productivity today
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.0 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.0 (0.2 micron filtered), 500 ml.
eMolecules Ambeed / N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic Acid / 5g / 600837773 / A332417 / / 29915-38-6 / MFCD00007538 / 243.270 / C7H17NO6S
Ambeed / N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic Acid / 5g / 600837773 / A332417 / / 29915-38-6 / MFCD00007538 / 243.270 / C7H17NO6S
Electron Microscopy Sciences Mops, Biological Buffer, 6.5-7.9 pH 100 GR
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged. Desired pH should be specified when ordering.
pH: 6.5-7.9
![Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60040-100.0.JPG-250.jpg)
Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 100 Grams
Tris Base Ultra Pure is a commonly used reagent and buffer component. Tris Base, Tris(hydroxymethyl)aminomethane, is a primary amine that acts as a buffering agent to maintain a stable pH in experimental procedures.
It is particularly effective in the pH range of 7.2 to 9.0, making it suitable for applications requiring slightly alkaline to mildly acidic pH conditions. Tris Base Ultra Pure is highly purified to remove impurities and contaminants, ensuring the highest quality for laboratory use.
The ultra-pure form is essential for applications where the presence of impurities can negatively impact experimental results. Tris Base Ultra Pure is commonly used in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzymatic assays.
It is also utilized in the preparation of electrophoresis buffers and PCR reactions.
Seracare Life Sciences Inc 10X TRIS BUFFERED SALINE (TBS)
10X Tris-buffered Saline (TBS) is used as a general wash buffer in immunoassay applications.
Teknova 1M Tris-HCl, pH 7.4. 1000 mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova TE Buffer, pH 8.0. (10mM Tris, 1mM EDTA). 250mL, Sterile, DNase/RNase Tested, PCR Grade.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.